If I am playing a game where I have a card in play that makes it so I don't lose from loss of life, and go to -1 life, would I need to gain 2 life in order to be safe without the anti-loss card anymore, or does the negative 'reset' to 0 after damage effects and cards on the stack resolve?
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You can go into negative life totals. If that doesn't cause you to lose, subsequent events still affect your life total normally: you could gain life back to zero or above, and losing more life will push you further negative.
The rulings on Beacon of Immortality confirm this:
So yes, if you're at -1 life, you'd need to gain 2 life to get back to 1 life and be safe without whatever was keeping you from losing.
You can go to negative life. So if you would go below 0, you keep tracking how much you've lost. If you are at 0 and lose 3, you will be at -3. If you then gain 2, you will be at -1, and would lose the game if you lose your effect that stops you from losing.